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Variability of the reverse transcription step: practical implications
journal contributionposted on 2023-07-26, 13:39 authored by Stephen A. Bustin, Harvinder S. Dhillon, Sara Kirvell, Christina Greenwood, Michael Parker, Gregory L. Shipley, Tania Nolan
The reverse transcription (RT) of RNA to cDNA is a necessary first step for numerous research and molecular diagnostic applications. Although RT efficiency is known to be variable, little attention has been paid to the practical implications of that variability.
Publication titleClinical Chemistry
PublisherAmerican Association for Clinical Chemistry