Variability of the reverse transcription step: practical implications
journal contribution
posted on 2023-07-26, 13:39authored byStephen A. Bustin, Harvinder S. Dhillon, Sara Kirvell, Christina Greenwood, Michael Parker, Gregory L. Shipley, Tania Nolan
The reverse transcription (RT) of RNA to cDNA is a necessary first step for numerous research and molecular diagnostic applications. Although RT efficiency is known to be variable, little attention has been paid to the practical implications of that variability.