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RT-qPCR Testing of SARS-CoV-2: A Primer

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posted on 2023-07-26, 14:58 authored by Stephen A. Bustin, Tania Nolan
Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.

History

Refereed

  • Yes

Publication title

International Journal of Molecular Sciences

ISSN

1422-0067

Publisher

MDPI

File version

  • Published version

Language

  • eng

Legacy posted date

2020-04-24

Legacy creation date

2020-04-24

Legacy Faculty/School/Department

COVID-19 Research Collection

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