Greenwood_et_al_2015.pdf (1.56 MB)
Download fileProximity assays for sensitive quantification of proteins
journal contribution
posted on 2023-07-26, 13:39 authored by Christina Greenwood, David Ruff, Sara Kirvell, Gemma L. Johnson, Harvinder S. Dhillon, Stephen A. BustinProximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein–protein interactions. Assays can be carried out in homogeneous or solid phase formats and in situ assays can visualise single protein molecules or complexes with high spatial accuracy. These properties highlight the potential of proximity assays in research, diagnostic, pharmacological and many other applications that require sensitive, specific and accurate assessments of protein expression.
History
Refereed
- Yes
Volume
4Page range
10-16Publication title
Biomolecular Detection and QuantificationISSN
2214-7535External DOI
Publisher
ElsevierFile version
- Published version
Language
- eng