Bustin_2017.pdf (912.95 kB)
How to speed up the polymerase chain reaction
journal contributionposted on 2023-07-26, 14:32 authored by Stephen A. Bustin
Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.
Publication titleBiomolecular Detection and Quantification
- Published version