Bustin_2017.pdf (912.95 kB)
How to speed up the polymerase chain reaction
journal contribution
posted on 2023-07-26, 14:32 authored by Stephen A. BustinReducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.
History
Refereed
- Yes
Volume
12Page range
10-14Publication title
Biomolecular Detection and QuantificationISSN
2214-7535External DOI
Publisher
ElsevierFile version
- Published version
Language
- eng
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Legacy posted date
2019-01-23Legacy creation date
2019-01-23Legacy Faculty/School/Department
ARCHIVED Faculty of Medical Science (until September 2018)Usage metrics
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