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Ex Vivo Mouse Aortic Ring Angiogenesis Assay

journal contribution
posted on 2025-03-21, 10:25 authored by Vedanta Mehta, Marwa Mahmoud
The ex vivo aortic ring assay is one of the most widely used protocols to study sprouting angiogenesis. It is a highly adaptable method that can be utilized to investigate the effects of different growth factors, small-molecule drugs, and genetic modifications on vascular sprouting in a physiologically relevant setting. In this chapter we describe a simple and optimized protocol for investigating vascular sprouting in the mouse aortic ring model. The protocol describes the harvesting and embedding of the aortic rings in a collagen matrix, treatment of the rings with agents of interest, and the visualization and quantification of the vascular sprouts.

History

Refereed

  • Yes

Volume

2475

Page range

229-238

Publication title

Methods in molecular biology (Clifton, N.J.)

ISSN

1064-3745

Publisher

Springer US

Location

United States

Language

  • eng

Item sub-type

Journal Article

Media of output

Print

Affiliated with

  • School of Allied Health Outputs