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Culture of Pulmonary Arterial Endothelial Cells from Pulmonary Artery Catheter Balloon Tips: Considerations for Use in Pulmonary Vascular Disease

journal contribution
posted on 2023-08-30, 16:48 authored by Corey Ventetuolo, Jason Aliotta, Julie Braza, Havovi Chichger, Mark Dooner, Donald McGuirl, Chris Mullin, Julie Newton, Mandy Pereira, Amy Princiotto, Peter Quesenberry, Thomas Walsh, Mary Whittenhall, James R. Klinger, Elizabeth O. Harrington
Endothelial dysfunction is a hallmark of pulmonary arterial hypertension (PAH) but there are no established methods to study pulmonary artery endothelial cells (PAECs) from living patients. We sought to culture PAECs from pulmonary artery catheter (PAC) balloons used during right heart catheterisation (RHC), to characterise successful culture attempts and to describe PAEC behavior. PAECs were grown in primary culture to confluence and endothelial cell phenotype confirmed. Standard assays for apoptosis, migration, and tube formation were performed between passage 3–8. We collected 49 PAC tips from 45 subjects with successful PAEC culture from 19 (39%) balloons. There were no differences in subject demographic or RHC procedural details in successful versus unsuccessful attempts. There was a higher but nonsignificant proportion of successful (10/19, 53%) versus unsuccessful (9/30, 30%) attempts from subjects who met hemodynamic criteria for PAH (p=0.10). Successful culture was more likely in subjects with lower cardiac index (p=0.03) and higher pulmonary vascular resistance (p=0.04). PAECs from a subject with idiopathic PAH were apoptosis resistant compared to commercial PAECs (p=0.04) and had reduced migration compared to PAECs from a subject with portopulmonary hypertension with high cardiac output (p=0.01). PAECs from a subject with HIV-associated PAH formed fewer (p=0.01) and shorter (p=0.02) vessel networks compared to commercial PAECs. Sustained culture and characterisation of PAECs from RHC balloons is feasible, especially in PAH with high hemodynamic burden. This technique may provide insight into endothelial dysfunction during PAH pathogenesis.

History

Refereed

  • Yes

Volume

55

Page range

1901313

Publication title

European Respiratory Journal

ISSN

1399-3003

Publisher

European Respiratory Society

File version

  • Accepted version

Language

  • eng

Legacy posted date

2019-12-11

Legacy creation date

2019-12-11

Legacy Faculty/School/Department

Faculty of Science & Engineering

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